PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Silva E.A., Natali M.R.M. & Prado I.M.M. 2008. The number and profile of reactive NADH-d and NADPH-d neurons of myenteric plexus of six-month-old rats are different in the cecum portions. Pesquisa Veterinária Brasileira 28(5):241-248. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, USP, Cidade Universitária, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-270. E-mail: elizangela@usp.br Whole-mount preparations were prepared and submitted to NADH-diaphorase and NADPH-diaphorase histochemistry techniques. The myenteric plexus arrangement and the number of neurons were comparatively evaluated among the different portions of the cecum. The neurons from the apical and basal regions were distributed in classes at intervals of 100µm2, the means of the corresponding intervals being compared. The ganglia, in both techniques, were often connected by fine bundles, which became thicker in the mesenteric region and in the region next to the cecal ampulla. The number of positive NADH-d neurons was higher than that of NADPH-d neurons in all portions, from both regions. The numbers of reactive NADH-d e NADPH-d neurons were significantly different among the different portions of the cecum, except for the antimesenteric basal and intermediate basal regions, considering the NADH-d neurons. The profile area for the reactive NADH-d e NADPH-d neurons was higher in the apical region than in the basal area. Differences in arrangement, distribution and size of positive NADH-d e NADPH-d neurons in the different cecum portions evidenced the importance of the subdivision of the analyzed organ.

• Myenteric neurons cecum portions cecum Wistar rats

Abstract in Portuguese:

ABSTRACT.- Silva E.A., Natali M.R.M. & Prado I.M.M. 2008. The number and profile of reactive NADH-d and NADPH-d neurons of myenteric plexus of six-month-old rats are different in the cecum portions. Pesquisa Veterinária Brasileira 28(5):241-248. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, USP, Cidade Universitária, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-270. E-mail: elizangela@usp.br Whole-mount preparations were prepared and submitted to NADH-diaphorase and NADPH-diaphorase histochemistry techniques. The myenteric plexus arrangement and the number of neurons were comparatively evaluated among the different portions of the cecum. The neurons from the apical and basal regions were distributed in classes at intervals of 100µm2, the means of the corresponding intervals being compared. The ganglia, in both techniques, were often connected by fine bundles, which became thicker in the mesenteric region and in the region next to the cecal ampulla. The number of positive NADH-d neurons was higher than that of NADPH-d neurons in all portions, from both regions. The numbers of reactive NADH-d e NADPH-d neurons were significantly different among the different portions of the cecum, except for the antimesenteric basal and intermediate basal regions, considering the NADH-d neurons. The profile area for the reactive NADH-d e NADPH-d neurons was higher in the apical region than in the basal area. Differences in arrangement, distribution and size of positive NADH-d e NADPH-d neurons in the different cecum portions evidenced the importance of the subdivision of the analyzed organ.

Abstract in English:

ABSTRACT.- Vidotti A.P., Agreste F.R., Bombonato P.P. & Prado I.M.M. 2008. [Arterial vascularization of the sinoatrial node in swine hearts: origin, distribution and quantification.] Vascularização arterial da região do nó sinoatrial em corações suínos: origem, distribuição e quantificação. Pesquisa Veterinária Brasileira 28(2):113-118. Departamento de Cirurgia, Setor de Anatomia dos Animais Domésticos e Silvestres, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-000, Brazil. E-mail: avidotti@usp.br The sinoatrial node, for being topographically installed as the initial component of the conduction system, is responsible for the production of the nervous impulses, which determines the cardiac contraction. There have been made studies related to the node’s morphology in order to know the origin, distribution and quantification of the vases in this tissue, however, in spite of the results and quantitative data of the nodal irrigation - arterial vascular conduct and arterial vascular density, at the nodal level - the literature is scarce. With this objective 27 SRD swine hearts, injected with colored resin for macroscopic analysis of the origin and distribution of ANSA (sinoatrial node artery), 3 others injected with watery solution of colloidal coal (dyed nanquim) were used, to mark the route of the vases on the node level for stereological analysis. The atrial arteries originated as well from the right coronary artery as from the left one, with predominance of the first (66.66% and 33.33%, respectively). When originated from the right coronary artery, there existed the following branches: AADAM (right cranial medial atrial artery) in 14 cases, AADAI (right cranial intermedial atrial artery) in 2 cases, and AADAL (right cranial lateral atrial artery) in 2 cases. In 9 cases (33.33%) the following branches originated from the left coronary artery: 4 through branch AASPL (left caudal lateral atrial artery), 2 through branch AASAI (left cranial intermedial atrial artery) and 3 through branch AASAM (left cranial medial atrial artery). Interarterial anastomoses with vessels responsible for irrigation of the sinoatrial node area were observed in most of the cases (25 hearts). The Volume or Reference Volume (V(ref)) of the hearts were 35.32x104µm3. For the stereological variables analyzed, the estimate density of the vessel length (Lv) was 766; the vessel length (L) - mm - was 27.06x105µm; the area’s surface density (Sv) was 182 and the area’s surface (S) - mm2 - was 64.3x106µm2. The estimate of the vascular numeric density (Nv(vasc)) - total of vessels per unit of volume (cm3) was 2.19 10-5, and the total number of vessels in the organ (N(vasc)), estimated by the physical dissector method in combination with the estimate of the number of Euler (Xv), was 773.6832 x10-2. The raised vascular density and total number of vases in the swine’s sinoatrial node area suggests the existence of a vascular perinodal network complex and dense, ratifying the importance of this mark-pass for its blood supply.

• Heart vascularization sinoatrial node morphometry swine

Abstract in Portuguese:

ABSTRACT.- Vidotti A.P., Agreste F.R., Bombonato P.P. & Prado I.M.M. 2008. [Arterial vascularization of the sinoatrial node in swine hearts: origin, distribution and quantification.] Vascularização arterial da região do nó sinoatrial em corações suínos: origem, distribuição e quantificação. Pesquisa Veterinária Brasileira 28(2):113-118. Departamento de Cirurgia, Setor de Anatomia dos Animais Domésticos e Silvestres, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-000, Brazil. E-mail: avidotti@usp.br The sinoatrial node, for being topographically installed as the initial component of the conduction system, is responsible for the production of the nervous impulses, which determines the cardiac contraction. There have been made studies related to the node’s morphology in order to know the origin, distribution and quantification of the vases in this tissue, however, in spite of the results and quantitative data of the nodal irrigation - arterial vascular conduct and arterial vascular density, at the nodal level - the literature is scarce. With this objective 27 SRD swine hearts, injected with colored resin for macroscopic analysis of the origin and distribution of ANSA (sinoatrial node artery), 3 others injected with watery solution of colloidal coal (dyed nanquim) were used, to mark the route of the vases on the node level for stereological analysis. The atrial arteries originated as well from the right coronary artery as from the left one, with predominance of the first (66.66% and 33.33%, respectively). When originated from the right coronary artery, there existed the following branches: AADAM (right cranial medial atrial artery) in 14 cases, AADAI (right cranial intermedial atrial artery) in 2 cases, and AADAL (right cranial lateral atrial artery) in 2 cases. In 9 cases (33.33%) the following branches originated from the left coronary artery: 4 through branch AASPL (left caudal lateral atrial artery), 2 through branch AASAI (left cranial intermedial atrial artery) and 3 through branch AASAM (left cranial medial atrial artery). Interarterial anastomoses with vessels responsible for irrigation of the sinoatrial node area were observed in most of the cases (25 hearts). The Volume or Reference Volume (V(ref)) of the hearts were 35.32x104µm3. For the stereological variables analyzed, the estimate density of the vessel length (Lv) was 766; the vessel length (L) - mm - was 27.06x105µm; the area’s surface density (Sv) was 182 and the area’s surface (S) - mm2 - was 64.3x106µm2. The estimate of the vascular numeric density (Nv(vasc)) - total of vessels per unit of volume (cm3) was 2.19 10-5, and the total number of vessels in the organ (N(vasc)), estimated by the physical dissector method in combination with the estimate of the number of Euler (Xv), was 773.6832 x10-2. The raised vascular density and total number of vases in the swine’s sinoatrial node area suggests the existence of a vascular perinodal network complex and dense, ratifying the importance of this mark-pass for its blood supply.

Abstract in English:

ABSTRACT.- Santos D.A.S., Ricci R.E.G., Prado I.M.M., Carvalho A.F., Bombonato P.P., Ambrósio C.E., Teixeira D.G., Santos T.C., H.-Brazquez F.J., Martins D.S., Morini A.C., Kfoury Jr J.R. & Miglino M.A. 2007. [Morphology and morphometry of buffalo mammary papillae.] Morfologia e morfometria das papilas mamárias de búfalas. Pesquisa Veterinária Brasileira 27(3):95-102. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: ceambrosio@usp.br Fifteen papillae from female water buffaloes and one bovine female were obtained in a slaughter house for this study, which consisted in morphologic and biometrical analyses, histology and histometric and mesoscopical analyses. The means were: right cranial papilla (RCP) 20.95g; right caudal papilla (RCaP) 21.49g; left cranial papilla (LCP) 23.52g; and left caudal papilla (LCaP) 21.35g. Papillary duct average length, numbers of folds and papillary cisterns length were, respectively: 0.67/5,5 e 2.17cm for RCP; 0.62/6.0 e 2.26cm for RCaP; and for the left antimere: RCP: 0.71/6.0 e 2.26cm and for RCaP: 0.74/5.7 e 2.57cm. Pearson’s analysis revealed negative correlation between body weight and mammary glandular weight. Sampling of proximal, middle and distal area was taken and fixed into a 10% formaldehyde solution, followed by mesoscopical investigation through 10, 20 and 40µm thickness cuts stained by Azan, F3BA Picrosirus and Weigert-Van Gieson techniques. Histological and morphometric analyses were applied on 5µm thick samples and showed that the muscular layer was disposed into two arrangements, internal circular and external longitudinal with a bi-stratified cuboidal epithelium. A well developed muscular layer were related to the middle portion revealing thickness of 22.90mm with a great amount of connective tissue (7.10mm).

• Buffaloes mammary papilla morphometry morphology Buballus bubalis

Abstract in Portuguese:

ABSTRACT.- Santos D.A.S., Ricci R.E.G., Prado I.M.M., Carvalho A.F., Bombonato P.P., Ambrósio C.E., Teixeira D.G., Santos T.C., H.-Brazquez F.J., Martins D.S., Morini A.C., Kfoury Jr J.R. & Miglino M.A. 2007. [Morphology and morphometry of buffalo mammary papillae.] Morfologia e morfometria das papilas mamárias de búfalas. Pesquisa Veterinária Brasileira 27(3):95-102. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP 05508-270, Brazil. E-mail: ceambrosio@usp.br Fifteen papillae from female water buffaloes and one bovine female were obtained in a slaughter house for this study, which consisted in morphologic and biometrical analyses, histology and histometric and mesoscopical analyses. The means were: right cranial papilla (RCP) 20.95g; right caudal papilla (RCaP) 21.49g; left cranial papilla (LCP) 23.52g; and left caudal papilla (LCaP) 21.35g. Papillary duct average length, numbers of folds and papillary cisterns length were, respectively: 0.67/5,5 e 2.17cm for RCP; 0.62/6.0 e 2.26cm for RCaP; and for the left antimere: RCP: 0.71/6.0 e 2.26cm and for RCaP: 0.74/5.7 e 2.57cm. Pearson’s analysis revealed negative correlation between body weight and mammary glandular weight. Sampling of proximal, middle and distal area was taken and fixed into a 10% formaldehyde solution, followed by mesoscopical investigation through 10, 20 and 40µm thickness cuts stained by Azan, F3BA Picrosirus and Weigert-Van Gieson techniques. Histological and morphometric analyses were applied on 5µm thick samples and showed that the muscular layer was disposed into two arrangements, internal circular and external longitudinal with a bi-stratified cuboidal epithelium. A well developed muscular layer were related to the middle portion revealing thickness of 22.90mm with a great amount of connective tissue (7.10mm).